| A total of 95 bacteria from six different epidemiologically important multilocus sequence types ST10, ST69, ST95, ST73, ST127 and ST131 were used in this project and principal component-discriminant function analysis PC-DFA of these samples produced clear separate clustering of isolates, based on the ST | However, whilst FT-IR spectroscopy is fast typically 10 s to 1 min per sample , the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism |
|---|---|
| The approach allowed separation of different isolates of Escherichia coli involved in urinary tract infection, including members of the globally disseminated ST131 clone, with respect to both genotype and resistance or otherwise to the antibiotic Ciprofloxacin | With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints |
Analysis of data using partial least squares-discriminant analysis PLS-DA , incorporating cross-validation, indicated a high prediction accuracy of 91.
.